Blotting membranes (transfer membranes) are used widely in biotechnology such as protein transers, western transfers, protein dot or slot blots, traditional DNA and RNA transfers, nucleic acid detection, northerns and southerns. Membrane Solutions affords all kinds of transfer membrane including PVDF transfer membrane, nitrocellulose blotting membrane and nylon blotting membrane. We can custom blotting membrane, like the shape, the size, the contents, the package and different use as per your requirements.
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PVDF Transfer Membranes |
MS® Life Science offers membranes for use in transfer and immobilization procedures. Selecting the appropriate membrane is critical to the success of a nucleic acid or protein transfer procedure. The several types of immobilon transfer membranes exhibit different performance characteristics which can directly affect the outcome of a specific technique.
| Bio-lott NC membrane | Bio-lott PVDF membrane |
Bio-lott Nylon |
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| Description | One of the most widely used membranes in analytical and research applications.
Minimize amounts of wetting agent and have a lower water extractable content.
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PVDF membrane has high protein adsorption, so proteins during transfer or reprobing won’t be lost. Open pore structure makes accessing bound proteins and removing unbound probes easily.
Membranes optimized for fluorescent blots dramatically increase signal for high sensitivity in quantitative, multiplexing applications.
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Open pore structure permits maximum accessibility of target sequences to the probe and allows efficient removal of unhybridized probe, thereby reducing background. |
| Composition | 100% Pure Nitrocellulose |
Polyvinylidene Fluoride |
Nylon-6 |
| Applications | Nucleic acid and protein transfers. Suitable for Western and Southern blotting. |
Western blotting, binding assays, amino acid analysis, N-terminal protein sequencing, dot/slot blotting, glycoprotein visualization, lipopolysaccharide analysis |
Colony/plaque lifts, dot/slot blotting, cell culture, clarification of aqueous solutions |
| Detection Methods | Chromogenic, Radioactive, Fluorescent |
Direct Stain, Enzyme-antibody Conjugates, Chromogenic, Chemiluminescent |
Chromogenic, Radioactive, Fluorescent |
| Typical protein binding capacity | 125ug/cm2 |
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| Compatible stains | Amido black, India ink, Ponceau-S red,Colloidal gold, CPTS |
Coomassie brilliant blue, Amido black, India ink, Ponceau-S red, Colloidal gold, CPTS, Toluidine blue, Transillumination, Sypro® ruby |
Amido black, India ink, Ponceau-S red, Colloidal gold, CPTS |
Works best for: Also suited for: |
Colony/Plaque Lifts Nucleic Acid and Protein |
Protein Transfers Nucleic Acid |
Protein Transfers Nucleic Acid |
| Advantages | Excellent strength No support fabric No detergents added 100% pure nitrocellulose |
Chemical resistance No discoloration Nonflammable High strength |
High strength High sensitivity Versatile adsorption properties Chemical resistance |
| Binding Interaction | Hydrophobic & Electrostatic |
Hydrophobic |
Hydrophobic |
Method of Immobilization |
UV Crosslink Baking (Vacuum Oven) |
Electroblott |
UV Crosslink Baking |
| USA | China | Japan |
| Toll Free: 1-866-528-4572 Fax: 732-412-4040 |
Toll Free: 400-820-0293 Fax: 0086-21-51687551 |
Toll Free: 0066-33-800658 |
