Blotting Membrane
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MS®Bio-lottTM Blotting membrane or Transferring Membrane is used widely in biotechnology, such as protein transfers, western transfers, protein dot or slot blots, traditional DNA and RNA transfers, nucleic acid detection, northern and southern.
Membrane Solutions affords PVDF blotting membrane, Nitrocellulose blotting membrane. We customize blotting membrane, like the shape, the size, the contents, the package for different use as per your requirements.
Guide to Select Blotting membrane
| NC Blotting Membrane | PVDF Blotting Membrane | |
| Composition | 100% Pure Nitrocellulose | Polyvinylidene Fluoride |
| Description | One of the most widely used membranes in analytical and research applications. Minimize amounts of wetting agent and have a lower water extractable content. | PVDF membrane has high protein adsorption, so proteins won’t be lost during transfer or re-probing. |
| Open pore structure makes accessing bound proteins and removing unbound probes easily. Membranes optimized for fluorescent blots dramatically increase signal for high sensitivity in quantitative, multiplexing applications. | ||
| Applications | Nucleic acid and protein transfers. Suitable for Western and Southern blotting. | Western blotting, binding assays, amino acid analysis, N-terminal protein sequencing, dot/slot blotting, glycoprotein visualization, lipopolysaccharide analysis |
| Detection Methods | Chromogenic, Radioactive, Fluorescent | Direct Stain, Enzyme-antibody Conjugates, Chromogenic, Chemiluminescent |
| Typical protein binding capacity | 125ug/cm2 | |
| Compatible stains | Amido black, India ink, Ponceau-S red,Colloidal gold, CPTS | Coomassie brilliant blue, Amido black, India ink, Ponceau-S red, Colloidal gold, CPTS, Toluidine blue, Transillumination, Sypro’s ruby |
| Works best for | Colony/Plaque Lifts | Protein Transfers |
| Suitable for | Nucleic Acid and Protein | Nucleic Acid |
| Advantages | Excellent strength | Chemical resistance |
| No support fabric | No discoloration | |
| No detergents added | Nonflammable | |
| 100% pure nitrocellulose | High strength | |
| Binding Interaction | Hydrophobic & Electrostatic | Hydrophobic |
| Method of Immobilization | UV Crosslink Baking (Vacuum Oven) | Electroblott |
Ordering Information
| Item # | Description | Pcs per box | Online Price(USD$) |
| NC Blotting Membrane | |||
| MSNC02030301 | Pore: 0.22μm, Filter Size: 300*3000mm | 1 | Ask for quote |
| MSNC04530301 | Pore: 0.45μm, Filter Size: 300*3000mm | 1 | Ask for quote |
| PVDF Blotting Membrane | |||
| MSPVDF02030301 | Pore: 0.22μm, Filter Size: 300*3000mm | 1 | Ask for quote |
| MSPVDF04530301 | Pore: 0.45μm, Filter Size: 300*3000mm | 1 | Ask for quote |
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