Solvent and Detergent Removal Chromatography Resin
The elimination of detergents and/or solvents from biological samples is often necessary and can be achieved by various methods including resin partitioning, size exclusion, affinity, or batch extraction with vegetable oils combined with reverse phase on C18 resin.
SDR HyperD resin is a composite sorbent that combines a silica-bead moiety filled with a three-dimensional cross-linked hydrophobic polymer. The SDR HyperD resin structure has been engineered to optimize solvent/detergent retention. The high specific surface area (200m2/g) of the porous silica allows a high binding capacity for detergent and solvents. The particle size distribution (40-100 μm), the small pore size of the silica beads, and the polymer have been optimized for retention of solvents and detergents used in viral inactivation processes (i.e., Tri-n-Butyl Phosphate (TnBP) and Triton♦ X-100)1. SDR HyperD resin is also very effective at removing detergents typically used in sample solubilization for other applications.
Triton X-100 interacts both with the silica surface (formation of hydrogen bonds between the silanols and the polyoxyethylene chain) and with the hydrophobic polymer moiety. TnBP interacts only with the hydrophobic polymer of the resin. The adsorption mechanism involves both the silica moiety and the hydrophobic polymer. The adsorption of Triton X-100 is proportional to the silica surface area, whereas the adsorption of TnBP is dependent on binding to the organic polymer moiety. SDR HyperD resin has proven useful for rapid detergent removal even when the detergent concentration is above the critical micellar concentration (CMC) and micelles are present. This is probably related to the equilibrium between micelle and free detergent molecules and/or a disruption of micelle structure on contact with the bead. The properties of SDR HyperD resin are summarized in Table 2.34 and the adsorption mechanism.