Tangential Flow Filtration (TFF) is a rapid and efficient method for separating and purifying biomolecules. Membrane Solutions’s TFF products combine low protein binding ultrafiltration or microfiltration membranes with optimized flow path design to quickly concentrate samples while achieving high concentration factors for sample volumes from 10 mL to thousands of liters. Systems are designed for easy set up and use. By incorporating the same path length and materials of construction throughout our TFF product line, conditions established during pilot-scale trials can easily be applied to process-scale applications.
Special Features Easy Set Up and UseSimply connect the TFF device to a pump and pressure gauge(s), add sample, and process.
High Concentration FactorsLow hold-up volumes allow high concentration factors to be achieved from small starting volumes.
Fast and Efficient ProcessingHigher concentrations can be achieved in less time than with centrifugal devices or stirred cells. Sample concentration and diafiltration can be achieved on the same system, saving time and avoiding product loss.
Scale Up or DownIdentical fluid path lengths and materials of construction allow precise linear scale-up to larger systems. The membrane area of a smaller device can be increased simply by connecting multiple devices or adding cassettes. Assuring predictable performance saves time when scaling a process from pilot to production.
EconomicalTFF devices and cassettes can be cleaned and reused, or disposed of after a single use. A simple integrity test can be performed to confirm that membrane and seals are intact. integrity test can be performed to confirm that membrane and seals are intact.
Applications Concentration and desalting proteins and peptides Protein fractionation Sample preparation prior to or post chromatography