UltraMem50 Crossflow Cassette

Membrane Solutions can provide single-use TFF solution, the UltraMem crossflow cassette, for your laboratory applications. UltraMem is a high performance tangential flow filter product with safety, convenience and cost efficiency for bio-pharm and bio-tech process. With the help of UltraMem, different types of lab scale bio products' concentration and separation can be achieve in lab effectively. Typical applications of UltraMem cassette include but no limited to the concentration and separation of monoclonal antibiotics, recombinant protein drugs, virus-vectors, vaccines, plasmid, mRNA LNP, particulate dispersions, etc.
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Related Documents

Tangential Flow UF Cassette User Manual

 

Features

  • UltraMem 50 is easy to use: plug, connect, ready to run
  • Expandable modular design: each UltraMemTM 50 cassette is an independent module and easy to connect with other modules. The expansion of multi-modules can linearly scale up the amount of liquid to be processed, up to 3000 ml
  • Innovative structural design: the specially designed inner flow path benefits the TFF process efficiency, lowers the shear force to fluid as well, friendly ro shear-sensitive liquids
  • Multiple high performance MWCO PES ultrafiltration membrane for you to choose
  • Safety: good integrity, low E&L and broadchemical compatiblity. Latest bio-pharm regulations USP 87 and 88 are met.

 

Applications

Purifications and concentration of:

  • mAB, recombinant proteins
  • Virus vectors and vaccines
  • Blood-derivative products
  • Plasmid, mRNA LNP
  • Particulate dispersions

Technical Parameters

 

Product
Information		 Device type TFF or Crossflow
L/H/W 115×90×24 mm
Effecive membrane area 50cm²
Materials of Construction Clamp Polypropylene
Housing material Polycarbonate
Seals & O-ring Medical Silicone
Membrane material Polyether sulfone (PES)
Operation Maximum allowable operating Temp. 60℃(140℉)
Maximum allowable(working)pressure 3bar(44psi)
Parameters Available MWCO 3/5/10/20/30/50/100 kDa
Volume can be Handled 100-3,000 ml
Min.Recirculating Volume 20 ml

 

FAQ

Q1: How to select the appropriate transmembrane pressure? 
A: Transmembrane pressure depends on feed rate, solution viscosity, membrane pore size, and restrictor adjustment. 
We recommend 1‒2 bar (max 3 bar). For first time use, start at 1 bar and increase gradually based on permeate flow.

 
Q2: What flow rate should be set for the peristaltic pump? 
A: Recommended flow rate: 200‒400 mL/min. Higher flow rates provide higher transmembrane pressure and better 
filtration efficiency. 


Q3: How to perform a water flux test? 
A: Set a suitable feed rate, adjust the restrictor to achieve 1 bar transmembrane pressure, measure the permeate 
volume (L), then divide by membrane area (m²) and time (h) to obtain LMH. 


Q4: How to maximize recovery of target molecules? 
A: After concentration, rinse the cassette and tubing with a small volume of buffer, then combine the rinse with the 
retentate. Typically >95% of the initial sample can be recovered. 


Q5: How to select the correct membrane pore size? 
A: For ultrafiltration concentration, choose an MWCO 3‒5 times smaller than the target protein (e.g., for a 150 kDa 
protein, use 30 kDa or 50 kDa cassette). For clarification (e.g., E. coli/yeast lysate, CHO cell culture media), choose 
0.22 μm or 0.45 μm. 


Q6: Should I run a single cassette or multiple cassettes in series? 
A: Use a single cassette for initial trials or sample volume ≤ 300 mL. For sample volumes up to 1 L or to increase 
processing speed, connect cassettes in series. 


Q7: Do I need extra accessories to connect two cassettes? 
A: No. Each box already includes the necessary tubing for series connection. 


Q8: Can the cassette be reused? 
A: Yes, but no more than 3 times is recommended. Actual reuse number depends on the sample.

 
Q9: Can the pump tubing be reused? 
A: Pump tubing wears out under peristaltic action. Replace every 2‒3 runs. 


Q10: How is the chemical compatibility? 
A: Stable in most common aqueous buffers and some solvents (e.g., 70% ethanol). See Section 3.4. 


Q11: How to handle leakage? 
A: Leakage occurs in two scenarios. Please refer to the following table based on the observed phenomenon.

Case 1 ‒ Target molecule appears in the permeate

Possible Cause Solution
Incorrect MWCO selection Choose an MWCO 3-5 times smaller than the target. For example:
Fora 150 kDa protein, a 30 kDa or 50 kDa cassette is recommended.
Chemical incompatibility damaging the membrane Referto the chemical compatibility table in Section 4 to confirm
whether the membrane material is compatible with the solution.
Over reused cassette, enlarged pores It is recommended to reuse each cassette no more than 3 times,
or replace it with a new cassette.

Case 2 ‒ Visible liquid seepage from the cassette housing

Possible Cause Solution
Clogged permeate outlet
causing pressure build up		 Carefully insert a syringe needle into the permeate outlet and gently clear the
blockage (be careful not to use excessive force to avoid damaging the cassette)
Loose Luer connections Use a clamp to appropriately tighten the connection.
being careful not to over tighten as this may cause cracking.
Pump tubing fatigue leading
to leakage at fittings		 It is recommended to replace the peristaltic pump tubing every 1-2 uses.
as tubing under prolonged pressure will lose its elasticity.