What is MS®Transfer Membrane?
MS® UltraTrans™ Transfer Membrane provides Polyvinylidene fluoride ( PVDF ) transfer membranes and Nitrocellulose ( NC ) transfer membranes.
Transfer membrane are widely used in biological detection and analysis, such as protein transfer, protein immunoblotting or protein western blot, traditional DNA and RNA transfer, nucleic acid hybridization detection etc.
Here Are Somethings More about MS® Transfer Membrane
Three Main Transfer Experiments:
Western Blot (WB) is a technique commonly used in research to isolate and identify proteins.
Unique vertical design and maximum filtra
Below WB experiment results show that Membrane Solutions transfer membrane has excellent performance
The Experimental parameters of MS® Transfer Membrane
Washing solution: TBST
Blocking: 5% defatted milk powder is blocked at room temperature for about 1 hour (shake slowly on the shakingtable)
Primary antibody: rabbit anti Escherichia coli antibody, rabbit anti BSA polyclonal antibody
Secondary antibody: Dilute the secondary antibody and incubate it on a shaking table at room
temperature for about 1 hour Development: MixECLchemiluminescence solution A and solution B in equal proportions and evenly drop them onto the membranefor exposure analysis using the Fusion imaging system
Wet transfer conditions: constant curent 400 mA, transfer for 40 mins.
2. How to select MS® Transfer Membrane according to application?
|
Application |
Membrane Type |
|
General Immunoassays |
MS® PVDF&NC 0.45μm |
|
Amino acid analysis |
MS ®PVDF 0.45μm |
|
Immunoassays for low abundance or small molecular weight proteins |
MS® PVDF&NC 0.22μm |
|
Sequencing of low abundance or small molecular weight proteins |
MS® PVDF 0.22μm |
3. What size we can provide to?
Except that, Membrane Solutions is launching convenient package Sandwich Membranes as well, which it hot type in market.
Conclusion
MS® Ultra Trans Transfer Membrane plays a valuable and efficient role in protein transfer, protein immunoblotting or protein western blot, traditional DNA and RNA transfer, nucleic acid hybridization detection etc.
For more detailed insights and inquiries about MS® Ultra Trans Transfer Membrane, please visit the membrane solutions website:
https://www.membrane-solutions.com/.
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