Paper chromatography is an analytical technique for separating and identifying mixtures that are or can be colored, especially pigments. Chromatography paper can also be used in secondary or primary colors in ink experiments. This method has been largely replaced by thin layer chromatography; however chromatography paper is still a powerful teaching tool.
Membrane Solutions' chromatography paper media are made from specially selected cotton cellulose. They are rigorously quality controlled for characteristics important to the chromatographer and to ensure uniformity within the grade. The chromatography paper product line includes standard cellulose and ion exchange grades. MS® chromatography paper’s good qualities are relied upon by chromatographers and are essential to successful, reproducible chromatography.
|Grade 1||The world standard chromatography paper. A smooth surface, 0.18 mm thick with a linear flow rate (water) of 130 mm/30 min. Good resolution for general analytical separations.|
|Grade 2||Thickness 0.18 mm. Flow rate 115 mm/30 min. Slower than 1 Chr for higher resolution applications. Smooth surface. Particularly recommended for optical or radiometric scanning.|
|Grade 3||A medium thickness paper (0.36 mm) with a flow rate of 130 mm/30 min. For general applications with medium/heavy solute loadings. Frequently used for separation of inorganic compounds and for electrophoresis.|
|Grade 3 MM||Though widely used as a blotting paper, 3MM Chr is also used both in electrophoresis and for general chemistry. A medium thickness paper (0.34 mm) used extensively for general chromatography and electrophoresis. Flow rate is 130 mm/30 min.|
|Grade 4||Thickness 0.21 mm. Flow rate 180 mm/30 min. Fastest of the thin papers. Recommended for routine and/or repetitive chromatography when loadings are relatively low. Smooth surface. Very suitable where speed is important and very high resolution is not required.|
|Grade 17||A thick (0.92 mm) and highly absorbent paper with a very high flow rate of 190 mm/30 min. Suitable for the heaviest loadings and ideal for preparative paper chromatography and electrophoresis.|
|Grade 20||Thickness 0.17 mm. Flow rate 85 mm/30 min. For maximum resolution, this grade is supreme, giving the greatest possible separation of closely related compounds. Smooth surface. Recommended for separation of samples of unknown composition, with outstanding resolution at low loadings.|
|Grade 31||Thickness 0.50 mm. Flow rate 225 mm/30 min. Extremely fast. Flow rate is the highest of all chromatography papers in the Whatman range. Thick paper with fairly soft surface. Principal application is in electrophoresis of large molecules.|