Get a quick overview of headspace sampling vials:bottle, septa and cap


Sample vials used for gas chromatography, headspace gas analysis are called headspace sample vials. Headspace sample vial is generally used for gas phase experiments. Although it is different in size from ordinary injection vials, its essence is similar to normal injection vials. The headspace sample vial is subdivided into 3 parts: bottle, septa and cap.


GC gas chromatography for analysis of volatile organic compounds in solid and liquid samples after gasification experiments. Especially for the detection of residual organic solvents in alcohols, blood, and pharmaceutical products.

Bottle Material

1.USP TYPE 1, Grade A, 33 borosilicate glass

The most chemically inert glass, widely used in laboratories, especially in chromatographic applications. Class I glass is mainly composed of silicon and oxygen, contains trace amounts of boron and sodium, and has the lowest solubility and a linear expansion coefficient of 33.

2.USP TYPE 1, B grade, 51 borosilicate hydrochloric acid glass

It is mainly composed of silicon and oxygen, contains trace amounts of boron, contains more alkali metals than A-grade glass, but is still suitable for laboratory use, and its linear expansion coefficient is 51.

3.Silanized or deactivated glass

Borosilicate hydrochloride glass, which has been deactivated by organosilylation, has a strong hydrophobicity and inertness on the glass surface, which is suitable for pH-sensitive compounds, trace analysis and long-term sample storage.

Bottle Options

Volume. Headspace sample vials have different capacity specifications, including 6ml, 10ml, 20ml, 40ml, etc. Use a standard sample vial to ensure sufficient headspace. Usually, the reserved volume should be at least 50% of the sample volume.

Color. Like most regular vials, the color of the headspace sample bottle is also different for us to choose: transparent and brown. Some samples that need to be stored in the dark can choose the brown one.

Bottom. Different from injection vials, the bottom of headspace sample vials is divided into round-bottom and flat-bottom, round-bottom headspace sample vials are easy to put into the heating module for heating, and flat-bottom vials are easy to place.

MS® HLPC GC Sample Vials provide advantages over the standard straight glass sample vials. The flared end of the vial makes loading a liquid sample much easier since the flare helps guide the syringe needle into the vial. The flared end also makes the vial much stronger so that vials can easily be inserted and removed from the end of the probe using tweezers without breaking the glass.

Cover pad structure

Single layer: usually made of red rubber or PTFE, for single use only;

Double layer: made of two different materials, one for the barrier (usually PTFE) and the other for the seal (usually silicone rubber);

Triple layer (also known as sandwich septum): Also made of two different materials, usually silicone rubber coated on both sides with PTFE.