MS® NC Transfer Membrane
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Transfer membranes are widely used in biological detection and analysis, such as protein transfer, protein immuno transfer, dot/slit hybridization, traditional DNA and RNA transfer, nucleic acid hybridization detection, etc. The Ultra Trans™ Nitrocellulose transfer membrane provided by Membrane Solutions has a high binding capacity for biomolecules, providing excellent sensitivity and low background. This transfer membrane is tailor-made for specific applications, detection, and blotting technologies and provides excellent performance. We can provide transfer membranes of different shapes and dimensions according to your needs for meeting your personalized needs.MS® Transfer membrane are purpose-built with features designed to bring the highest levels of performance and purity to your research. We incorporate a variety of membranes to offer separation and purification solutions for the majority of your laboratory needs. Selecting the appropriate membrane is critical to the success of a nucleic acid or protein transfer procedure.
Feature
- Excellent mechanical strength
- High chemical compatibility
- Excellent sensitivity
- Low background
Application
- Western Blot
- Southern Blot
- Northern Blot
- Amino acid or protein analysis
Article Number and Specification
| Article No | Specifications | Package |
| MSNCO2010011 | Pore: 0.22um, Filter Size: 100*100mm | 25 pcs |
| MSNCO2015015 | Pore: 0.22um, Filter Size: 150*150mm | 25 pcs |
| MSNC02030301 | Pore: 0.22um, Filter Size: 300*3000mm | 1 roll |
| MSNC02030401 | Pore: 0.22um, Filter Size: 300*4000mm | 1 roll |
| MSNCO4510011 | Pore: 0.45ym, Filter Size: 100*100mm | 25 pcs |
| MSNCO4515015 | Pore: 0.45um, Filter Size: 150*150mm | 25 pcs |
| MSNC04530301 | Pore: 0.45um, Filter Size: 300*3000mm | 1 roll |
| MSNC04530401 | Pore:0.45um, Filter Size:300*4000mm | 1 roll |
Attached: Nitrocellulose transfer membranes' structure under electronic microscope
Attached: WB experiment result
Note: Target protein GADPH, results 1-3 are from three batches of 0.45um NC transfer membrane, results 4-6 are from three batches of 0.22um NC transfer membrane.
Experimental Parameters
Washing solution: TBST;
Blocking: Block with 5% skimmed milk powder at room temperature and shake slowly on a shaker for about 1 hour;
Primary antibody: dilute the primary antibody with antibody diluent, and incubate on a shaker at 4°C overniaht:
Secondary antibody: dilute the secondary antibody at a ratio of 1:5000, and incubate on a shaker at room temperature for about 1 hour.
Development: Mix ECL chemiluminescence liquid A and liquid B in equal proportions, drop them evenly onto the membrane, and expose and analyze with the Fusion imaging system;
Transfer conditions: constant curent 200mA, wet transfer 60 min.