Centrifugal Filter

RevoSeizer Centrifugal Filter device can quickly and effectively concentrate and purify biological samples, and currently offers specifications of 0.5ml, 4ml, and 15ml. The unique vertical design and maximum filtration area provide fast sample processing and high sample recovery rate (typically greater than 90% of dilute starting solution), while maintaining a gentle concentration environment to preserve protein activity and conformation. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss. The wide selection of ultrafiltration molecular weight cut-off (MWCO) devices incorporate PES membrane, which is very low in protein and nucleic acid binding.
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Related Documents

MS RevoSeizer Centrifugal Filter Datasheet MS RevoSeizer Centrifugal Filter Quick Usage Guide

  

Features

  • Precise Retention, low protein adsorption, high recovery rate >90%
  • Dual-Sided Vertical Membrane Design. Prevents membrane clogging, ultra-low holdup volume, high centrifugal efficiency
  • Excellent Biocompatibility& Safety. Minimal extractables, USP87 certified
  • Anti-Dry Filtration Locking Design.Prevents over-centrifugation to ensures stable and reliable experimentalresults
  • Diverse Membrane Options
  • OEM customization

Applications

The filtration process is carried out by depth filter sheets, which are available in a range of porosities, from coarse to fine, and even germ-reducing filtration.
 
Ultra-filtration centrifugal filter:

  • Concentrate and desalt proteins, nucleic acids
  • Buffer exchange or desalting of chromatographi components
  • Harvest bio-molecules from culture media
  • Virus concerntration or isolation
  • Coarse separation of bio-molecule mixtures
  • Removal of debris and particles from cell lysates

Micro-filtration centrifugal filter:

  • Isolate DNA from agarose gel
  • Separation of proteins, oligonucleotides and RNA from polyacrylamide gels
  • Sample clarification before HPLC analysis
  • Media removal of cells
  • Filter biological samples
  • Collect and clean processed particles or beads
  • Fill chromatographic media for analytical procedures or process development

Technical Parameters

Product Cat 0.5ml 4ml 15ml
Filter PC(Polycarbonate) SBC(Styrene buta diene copolymer)
Membrane Polyethersulfone
Filtrate Tube Polypropylene
Filtrate Cap and Liner Polypropylene
Effective Filter Area 0.9cm² 3.5cm² 7.2cm²
Filters in Test Tubes
(Capped)

Overall lengt(concentrated   mode:filter inserted into outer tube):50.3mmOverall lengt(reverse centrifugation:
filter inserted backwards into outertube):46.8mm
Diameter:10.9mm

 Overall length  122mm
Diameter 17mm		 Overall length  117mm
Diameter31mm
Filter Length:29.2 mm Diameter:9.4 mm Length:67.5mm Diameter:14.5mm Length:75mmDiameter:28mm
Retention Volume ≤5μl ≤20μl ≤30μl
Locking Volume 10-20μl 50-100μl 300μl
Operating Temperature 0-40℃
pH Range 1-14
Maximum     Centrifugal
Force(Ultra Filtration)		 14000x g Swing bucket rotor4000x g Swing bucket rotor 4000x g
      (100KD 3000x g)
Fixed angle rotor 6000x g Fixed angle rotor5000xg
   (100KD 3000x g)
Maximum     Centrifugal
Force(Micro Filtration)		 / Microfiltration:10000x g Microfiltration:6000x g
Centrifuge Type Centrifuge standard 1.5ml conical tubes Centrifuge standard
15 ml conical tubes		 Centrifuge standard
50 ml conical tubes
Sterilization Non-sterile,can be disinfected and sterilized by filtering 70% ethanol through the equipment.

FAQ

Product Knowledge

1.What are the uses of RevoSeizerTM Centrifugal Filters?

RevoSeizerTM centrifugal filters can be used for concentration, desalting, or buffer exchange of biological samples (proteins, nucleic acids, viruses, etc.).

2.What specifications are available for RevoSeizerTM Centrifugal Filters?

RevoSeizerTM centrifugal filters are offered in three sample volume specifications: 0.5 mL, 4 mL & 15 mL. Each specification includes five nominal molecular weight cut-offs (MWCO): 5 kDa, 10 kDa, 30 kDa, 50 kDa, 100 kDa. For processing larger volume samples, please consult technical support.

3.What is the membrane material of RevoSeizerTM Centrifugal Filters?

The membrane material of RevoSeizerTM centrifugal filters is PES (Polyethersulfone).

4.What is the chemical compatibility of RevoSeizerTM Centrifugal Filters?

RevoSeizerTM centrifugal filters are suitable for general aqueous solutions or biological samples.For the chemical compatibility of specific samples, please refer to the chemical compatibility table in this manual, or send us an email: info@membrane-solutions.com

5.Can I reuse the Centrifugal Filters?

All centrifugal filters are disposable and reuse is not recommended.

6.What is the tolerable pH range for the Centrifugal Filters?

For the PES ultrafiltration membrane, the recommended pH range in the instructions is 1-14. For specific acid/base types and concentrations, please refer to the chemical compatibility table in the instructions.

 

Product Selection

1.How do I choose the appropriate RevoSeizerTM device for protein concentration?

Generally, we recommend selecting an centrifugal filters with a nominal MWCO less than or equal to 1/3 of the target protein's molecular weight.

2.If using ultrafiltration to separate two proteins, how much should their sizes differ?

Based on our experience, we recommend the molecular weights of the two proteins differ by an order of magnitude (10 times).

3.Can RevoSeizerTM Centrifugal Filters be used for virus concentration?

Yes. For lentivirus, we recommend RevoSeizerTM 100 kDa MWCO; for adenovirus, RevoSeizerTM 50 kDa MWCO is recommended.

4.Can RevoSeizerTM Centrifugal Filters be used for nanoparticle purification and concentration?

Yes. Although we lack internal data to support this application, many customers use RevoSeizerTM centrifugal filters for nanoparticle concentration and purification with good results.Please refer to the chart below for the membrane pore size range corresponding to the nominal MWCO of RevoSeizer™ centrifugal filters：

Nanoparticle Diameter(DIA)

Recommended MWCO

1.5<dia<3nm

3,000

3<dia<5nm

10,000

5<dia<7nm

30,000

7<dia<10nm

50,000

10<dia

100,000

5.Can RevoSeizerTM Centrifugal Filters be used for exosome purification and concentration?

Yes. RevoSeizerTM centrifugal filter with 50-100kDa is recommended

 

Experimental Operation

1.What kind of centrifuge rotor should I use with RevoSeizerTM Centrifugal Filters, and what is the maximum centrifugal force?

Please refer to the following table for specific parameters:

2.How is the processed sample collected?

Two methods: Pipette transfer and Reverse Spin.

(1) For 15 mL and 4 mL centrifugal filters, the processed sample can be collected directly using a pipette.

(2) For 0.5 mL centrifugal filters, the processed sample can be collected by reverse spin: Invert the filter device (inner tube) containing the concentrated sample into a new collection tube, then centrifuge at low speed (1,000xg) to transfer the sample into the new collection tube (steps shown in the figure below).

3.Will the sample in the RevoSeizerTM Centrifugal Filters dry out if centrifuged too long?

No, because RevoSeizerTM has a dead volume design, some solution will always remain in the filter device.

4.Can RevoSeizerTM Centrifugal Filters be sterilized with alcohol?

RevoSeizerTM devices are compatible with 70% ethanol. However, we have not tested specific sterilization methods and therefore cannot provide further reference information.

5.Can RevoSeizerTM ultrafiltration devices be autoclaved?

No.

6.Does Membrane Solutions provide endotoxin-free ultrafiltration devices?

The ultrafiltration devices we provide are not endotoxin-free. Even sterile devices may contain pyrogens.

7.Are RevoSeizerTM Centrifugal Filters RNase-free?

We do not guarantee that the centrifugal filters are RNase-free. It is recommended that you treat them with 0.1% DEPC soaked at 37°C for 2 hours to completely inactivate RNases. Residual DEPC can be removed by washing with ultrapure water.

8.The manual recommends centrifugation at room temperature. Considering protein stability, can I centrifuge at 4℃?

Yes, but low temperature increases the viscosity of protein samples, leading to slower flow rates. It is recommended to extend the centrifugation time to 1.5 times the original duration.

9.How to perform endotoxin removal treatment on the ultrafiltration centrifugal tubes?

The centrifugal filters we provide have not undergone endotoxin removal treatment.Furthermore, since endotoxins often exist as polymers with sizes ranging from 10-1000 kDa, they cannot be removed during the ultrafiltration process.

Result Analysis

1.Are there any precautions when using RevoSeizerTM Centrifugal Filters to remove detergents?

Due to their unique properties, when the concentration is above the Critical Micelle Concentration (CMC), detergent molecules aggregate to form micelles, changing their molecular conformation. This may affect the efficiency of detergent removal.

2.Why did my protein precipitate during concentration?

Protein precipitation can be caused by too rapid concentration or over-concentration. We recommend that the final concentration of the protein after concentration does not exceed 20 mg/mL. For proteins sensitive to concentration speed and prone to precipitation, we suggest the following improvements:

1) Reduce the centrifugal force to 30%-50% of the recommended force.

2) Switch to the next larger MWCO device (e.g., if 10 kDa was originally selected, try 30 kDa).

3) During concentration, remove the centrifugal filters and pipette the sample up and down several times.

3.After concentration, I found no target protein in the concentrate. What could be the reason?

First, the minimum starting protein concentration for RevoSeizerTM centrifugal filters is 25 µg/mL. Please ensure your sample's starting concentration is greater than this. Second, if the problem persists, please do not discard your sample filtrate for further analysis:

1) If your sample is in the filtrate, then check:

- Did you choose the appropriate MWCO ultrafiltration centrifugal tube (MWCO <= 1/2 or 1/3 of the target protein molecular weight)?

- Was the centrifugal force within the maximum range? If you used rpm, convert it to the corresponding RCF based on the centrifugal radius, or send us an email:info@membrane-solutions.com

- Has the centrifuge been calibrated recently?

- Is this your first attempt with this protein? If you can ensure the UF tube works for other proteins, then it might be due to your target protein.

Sometimes, protein characteristics (conformational differences) can affect concentration efficiency. Try selecting a smaller MWCO UF tube (e.g.,if 30 kDa was originally selected, try 10 kDa).

2) If your sample is not in the filtrate either:

- Was your starting protein concentration greater than 25 µg/mL?

- What method did you use to determine the sample concentration? Is it reliable?

- Did your target protein precipitate? If so, please refer to the FAQ above regarding protein precipitation for specific solutions.

4.I found interference when analyzing the concentrated protein. What could be the cause?

The ultrafiltration membrane in RevoSeizerTM centrifugal filters contains trace amounts of glycerol. If this material interferes with the analysis, pre-rinse with buffer or ultrapure water. If interference persists, rinse with 0.1 mol/L NaOH, then rinse again with buffer or ultrapure water and spin dry.

5.Sometimes I can't even spin water through the UF tube. What could be the reason?

The ultrafiltration membrane in RevoSeizerTM centrifugal filters contains trace amounts of glycerol. If this happens, first rinse with 0.1 mol/L NaOH and then centrifuge. Finally, rinse again with buffer or ultrapure water and spin dry. The cleaned membrane should be used immediately. If not used temporarily, keep it moist to avoid re-drying.

6.I suspect non-specific adsorption between the target protein and the membrane during concentration using the UF tube. How can I improve this?

The UF tube uses PES membrane material, which has low protein adsorption. However, for some hydrophobic or non-polar proteins, non-specific adsorption to the membrane may be enhanced. For this situation, customers can try blocking the UF tube before the experiment. For detailed steps, please contact technical support. It is also recommended to reduce the membrane area to minimize non-specific adsorption.